solexa
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| solexa [2007/12/20 13:12] – heidi | solexa [2008/07/22 13:31] (current) – external edit 127.0.0.1 | ||
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| Here is a sketch of the procedure I used. The input data consists of: | Here is a sketch of the procedure I used. The input data consists of: | ||
| - | - the reference DNA sequence of each exon, usually flanked by 20 nt from the surrounding introns | + | - the reference DNA sequence of each exon, usually flanked by 20 nt |
| + | from the surrounding introns | ||
| - the roughly 4.5 million Solexa 35 nt reads | - the roughly 4.5 million Solexa 35 nt reads | ||
| - the NCBI reference human genome | - the NCBI reference human genome | ||
| Line 35: | Line 36: | ||
| are not penalizing) | are not penalizing) | ||
| - all the reads from C are searched for perfect matches in the | - all the reads from C are searched for perfect matches in the | ||
| - | reference genome using fetchGWI[1], | + | reference genome using fetchGWI[1], |
| - | >from the one that find a perfect match outside of the position of the | + | from the one that find a perfect match outside of the position of the |
| exon under consideration on the reference genome | exon under consideration on the reference genome | ||
| - all the reads from C are aligned to the concatenated sequence | - all the reads from C are aligned to the concatenated sequence | ||
solexa.1198152770.txt.gz · Last modified: 2008/07/22 13:30 (external edit)