Masterarbeit, Heidi Lischer

User Tools

Site Tools

Trace: • 03.03.2008
fastq

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
fastq [2011/09/05 15:27] – heidifastq [2011/09/19 16:19] (current) – heidi
Line 10: Line 10:
   * text based   * text based
   * no standard file extension, but .fq, .fastq, and .txt are commonly used.   * no standard file extension, but .fq, .fastq, and .txt are commonly used.
 +
  
  
Line 20: Line 21:
   * A FASTQ file normally uses four lines per sequence:   * A FASTQ file normally uses four lines per sequence:
     * Line 1: begins with a '@' character and is followed by a sequence identifier and an optional description (like a FASTA title line)     * Line 1: begins with a '@' character and is followed by a sequence identifier and an optional description (like a FASTA title line)
-    * Line 2: is the raw sequence letters+    * Line 2: is the raw sequence letters (IUPAC ambiguity codes: ACTGNURYSWKMBDHV)
     * Line 3: begins with a '+' character and is optionally followed by the same sequence identifier (and any description) again.      * Line 3: begins with a '+' character and is optionally followed by the same sequence identifier (and any description) again. 
     * Line 4: encodes the [[http://phd.chnebu.ch/index.php/Next-Generation_Sequencing_data|quality values]] for the sequence in Line 2 and must contain the same number of symbols as letters in the sequence.     * Line 4: encodes the [[http://phd.chnebu.ch/index.php/Next-Generation_Sequencing_data|quality values]] for the sequence in Line 2 and must contain the same number of symbols as letters in the sequence.
fastq.1315229267.txt.gz · Last modified: 2011/09/05 15:27 by heidi